|Year : 2022 | Volume
| Issue : 1 | Page : 12-17
Histopathological spectrum of benign lesions of the breast from a university teaching hospital in Northern Nigeria
Murtala Abubakar1, Balarabe Kabir1, Almustapha Aliyu Liman1, Sani Mohammed Shehu1, Saad Aliyu Ahmed1, Peter Pase Abur2, Mohammed Bashir2, Zainab A Adamu1, Kamarudeen Owolabi Sani1, Murphy Ibiam Osita-Ufere3, Shehu Abdullahi1, Aliyu Usman Bappa1
1 Department of Pathology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria
2 Department of Surgery, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria
3 Department of Pathology, Nmandi Azikwe University Teaching Hospital, Nnewi, Anambra State, Nigeria
|Date of Submission||13-Nov-2021|
|Date of Decision||25-Dec-2021|
|Date of Acceptance||25-Dec-2021|
|Date of Web Publication||3-Aug-2022|
Dr. Murtala Abubakar
Department of Pathology, Ahmadu Bello University Teaching Hospital, Zaria
Source of Support: None, Conflict of Interest: None
Background: Benign breast diseases (BBDs) are a term that refers to a heterogeneous group of lesions of the breast that are not malignant. They are more common than malignant breast lesions and are important because of the need to differentiate them from breast cancer and for the increased risk of cancer development associated with some behavior-driven development. Aims: The aim of this work was to classify and study the clinicopathological patterns of BBDs as seen in our hospital. Methodology: All benign breast lesions diagnosed in our over the study period were studied. Data were extracted from patients' request cards, hematoxylin- and eosin-stained slides, and histopathology reports issued for each of the cases. The data were analyzed and presented in tabular formats. Results: Four hundred and fiftyfour cases of BBDs were recorded over the study period. 96% of these cases were reported in the female patient. The mean age of our patients was 29.62 ± 8.86 years with the age group of 21–30 years having the highest frequency. Fibroadenoma (FA) (226 cases) was the most common followed by fibrocystic diseases (FCDs). Four cases of atypical ductal hyperplasia were seen. Lesions of the male breast were seen in 18 patients and all were cases of gynecomastia. Conclusion: BBDs are the most common histologically diagnosed breast diseases in our center. FA, and FCDs were the most common BBDs. A relatively low prevalence of premalignant lesions not reflective of the high incidence of breast cancer in our environment was observed. Routine mammographic screen for these lesions is therefore recommended.
Keywords: Benign, breast diseases, northern Nigeria
|How to cite this article:|
Abubakar M, Kabir B, Liman AA, Shehu SM, Ahmed SA, Abur PP, Bashir M, Adamu ZA, Sani KO, Osita-Ufere MI, Abdullahi S, Bappa AU. Histopathological spectrum of benign lesions of the breast from a university teaching hospital in Northern Nigeria. Ann Trop Pathol 2022;13:12-7
|How to cite this URL:|
Abubakar M, Kabir B, Liman AA, Shehu SM, Ahmed SA, Abur PP, Bashir M, Adamu ZA, Sani KO, Osita-Ufere MI, Abdullahi S, Bappa AU. Histopathological spectrum of benign lesions of the breast from a university teaching hospital in Northern Nigeria. Ann Trop Pathol [serial online] 2022 [cited 2022 Oct 3];13:12-7. Available from: https://www.atpjournal.org/text.asp?2022/13/1/12/353202
| Introduction|| |
A wide number of benign and malignant lesions have been reported in the breast, although it is the malignant lesions that mostly grab attention and headlines due to their higher burden in terms of mortality and morbidity.
Benign breast diseases (BBDs) constitute a heterogeneous group of conditions ranging from congenital/developmental, inflammatory, proliferative and benign neoplastic lesions of the epithelium and/or stroma, as well as the nipple/skin covering of the breast.
Worldwide, BBDs are reported to be more common than malignant breast lesions and are important because of the need to differentiate them from breast cancer; the documented increased risk of cancer development associated with some of behavior-driven development (BDD), and the morbidity associated with them.
These lesions have been reported to be common worldwide and are seen more commonly in females than men. Age distribution of BDDs varies among the various entities, but as a group, the incidence has been reported to begin to rise in the second decade of life and peaks in the third and fourth decades. Hormonal influence on the breast has been proposed to be of etiologic significance in the development of these conditions.
Clinically, patients with BBDs seek medical care mostly because of a palpable mass, a diffuse breast swelling/pain or a nipple discharge. These presenting features often mimic cancer, thereby generating anxiety which is further worsened in our resource constrained setting by a lack of readily available and reliable means of screening/early detection. Furthermore, it has been established that some forms of BDDs are forerunners to the development of cancer as evidence by the multifold increase in cancer risk associated with them.
Although, a sizable number of studies on breast masses had been carried out in Nigeria including a 19-year-old study from our hospital, some of them have a more clinical rather than morphological inclination. Furthermore, the need to observe changing trends, if any, adds to the justification of the index study.
The aim of this work was to classify and study the clinicopathological patterns of BBDs as seen in our hospital.
| Methodology|| |
This s a retrospective analysis of all BBDs diagnosed in the Histopathology Department of a university teaching hospital in Northern Nigeria over a 5-year period (January 2015–December 2019).
The cases of interest were extracted from the surgical daybooks of the years under review. Other relevant data were gotten from the pathology request forms filled by the attending physicians and copies of the pathology reports issued on all the cases. The corresponding glass slides for all cases were retrieved from archives. In cases of missing slides, new ones were cut from formalin fixed, paraffin-embedded tissue blocks. Each diagnosis was verified by a consensus of 2 consultant pathologists. All slides examined were 3–5 μm thick sections made from 10% buffered formalin preserved tissue, processed according to standard protocols, and embedded in paraffin wax. The primary stain employed in all cases was hematoxylin and eosin. A few inflammatory lesions were stained with Ziehl–Neelsen and Grocott-Gomori's Methenamine Silver Stains for acid-fast bacilli and fungal organisms, respectively.
Cases for which both the glass slides and the tissue blocks are missing were excluded from the study.
The resulting data were subjected to simple descriptive statistical methods using Microsoft Excel statistical package.
Ethical clearance for the study was obtained from the Health Research Ethics Committee of our hospital.
| Results|| |
Four hundred and fifty-four cases of BDDs were recorded in our center over the 5-year study period. They constituted 72.1% of the 631 breast specimens received in the laboratory (malignant to benign ratio was 1:1.4). Four hundred and thirty-six of the cases (96%) were recorded in females, while the remaining 18 cases were seen in male patients giving a male-to-female ratio of 1:24.
Our patients were within the age range of 12–68 with a mean of 29.62 ± 8.86 years. The age group with the highest frequency of BDD was 21–30 years (173 cases), while the least frequency (4 cases) was seen in patients aged 61–70 years. [Table 1] presents the age distribution of the patients.
Fibroepithelial lesions, seen in 331 patients (72.91% of cases), constituted the most common category of the lesions seen in our patients. The least common category was lesions of the nipple with 2 cases (0.44%) seen. [Table 2] summarizes the frequency distribution of all the lesions seen.
Within the fibroepithelial category of lesions, fibroadenoma (FA) with 226 cases (68.3% of this category) was the most common, followed by fibrocystic diseases (FCDs) (30.21%). Six of these cases of FCDs were high-grade lesions atypical ductal hyperplasia (ADH) with greater propensity for malignant transformation. Five cases of benign phyllodes tumor (1.1% of all BDD) were also recorded within the study period [Table 2]. [Figure 1], [Figure 2], [Figure 3] show photomicrographs of FA fibrocystic changes and benign phyllodes tumor respectively.
|Figure 1: Photomicrograph showing a biphasic tumour composed of proliferating benign ductal elements as well as a proliferating myxoid intralobular stroma (H and E, ×40)|
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|Figure 2: Photomicrgraph showing a lesion composed of areas of adenosis, cyst formation and fibrosis. Mild epitheliosis and apocrine metaplasia are also present|
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|Figure 3: Photomicrograph showing benign ductal elements in a stroma exhibiting leaf like overgrowth. No significant mitotic activity. No stromal atypia (H and E, ×40)|
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The mean age for patients with FA and FCDs were 23.2 and 33.6, respectively. Age ranges for patients with FA and FCDs were 14–38 and 18–58 years, respectively. Over half of our patients (53.1%) diagnosed with FA were in the age range of 21–30 years, while the age group with the highest frequency of FCDs was 31–40 years (44% of the cases). [Table 3] gives the age distribution of the histological entities under the fibroepithelial category of lesions.
Inflammatory diseases of the breast were diagnosed in 43 patients constituting 9.4% of the BBDs seen within the study period. Chronic nonspecific mastitis and fat necrosis were the most common entities seen under this category with frequencies of 14 and 12 cases, respectively. Ten cases of granulomatous mastitis were also seen out of which, 2 cases were confirmed as tuberculous mastitis upon positive staining with Ziehl–Nielsen (ZN) stain, while the remaining 8 cases stained negative to both ZN and fungal stain (Gomori Methenamine Silver).
Fibrous change (8 cases) and pseudoangiomatous stromal hyperplasia (5 cases) were the common stromal breast lesions seen among our subjects. Other lesions diagnosed in this category were fibrolipoma (4 cases) and granular cell tumor (1 case).
Tubular adenoma and intraductal papilloma were the most common benign epithelial lesions of the breast seen among our patients. These two entities constituted 12 (50%) and 6 (25%) of the benign epithelial lesions of the breast, respectively. Most of the patients diagnosed with tubular adenoma were in the 21–30-year age range with a mean age of 29.67 ± 6.69 years [Table 4].
Lesions of the male breast were seen in 18 (4%) of BBD cases. All cases seen in this category were that of gynecomastia. Majority of the cases were seen in patients above 50 years of age, with a mean age of 47.2 ± 5.4 years [Table 5].
| Discussion|| |
BBDs are a group of noncancerous disorders of the breast which can occur in both men and women. The spectrum of lesions belonging to this category includes developmental abnormalities, inflammatory disorders, nonneoplastic proliferations, and neoplastic conditions of the breast.
In our study, 454 cases of BBD were seen, constituting 71.9% of the 631 breast tissue samples handled in our laboratory over the 5-year study period. This is consistent with a previous report from our center (71.3%) and with other Nigerian reports from Kano, Calabar, and Benin. However, a slightly higher proportion of BBDs (90%) was reported by Adeniji et al. from Ife, while Mayun et al. reported a bit lower proportion (59.5%) from Gombe, all in Nigeria. In all these reports, meanwhile, BBDs constituted a clear majority of diagnosed breast lesions similar to what was found among Kenyan (72%), and Afro-Caribbean populations in Jamaica (80%). Globally, BBDs are reported to constitute 90% of all breast lesions.
The index study revealed a 96% of BBD cases are recorded in female patients (M: F = 1:24). This collaborates local,,, regional, and global reports. It was postulated that the higher breast volume, the more complex architecture, and the repetitive cyclical hormonal influences in the female breast might be contributory to this skewed distribution.
The highest frequency of BBDs was seen among women between the ages of 21–30 years, with the mean age being 29.62 ± 8.86 years. Similar age distribution was reported in Nigeria by Ngadda et al. and Imam et al. Reports from other centers in Africa,, and other parts of the world,, have all revealed a similar pattern.
FA was the most common BBD seen in our study. It constituted 49.8% of the cases. Several previous reports agree with this finding. Notably, Imam et al.; Ngadda et al., Echejoh et al., all in Northern Nigeria, as well as reports from other Nigerian,, and African centers, are all corroborative on this. In all aforementioned studies, FCDs ranked as the most common BBD. Meanwhile, two Nigerian studies from Kano and Ibadan reported a reversed situation in which FA ranked 2nd with FCD in 1st position. However, it is noteworthy that those studies were 19 and 48 years old, respectively. It might be that what we are having now is a changed pattern as postulated by Aisha et al.
The mean age of patients with FA was 23.2 years (age range: 14–38 years). Other reports include mean ages of 23.52 years from Uyo, 23.1 years from Bayelsa, and 22.9 years from Benin, Nigeria. Similarly, over half (53.3%) of our patients with FA were in the age range of 21–30 years with a sharp decline in frequency seen from the fourth decade of life upward. This trend is similar to that observed among Nigerians, Africans, and Caucasians.
FCDs consist of a spectrum of morphologic changes comprising varying degrees of cyst formation, adenosis, epitheliosis, apocrine metaplasia, and fibrosis. The fact that these changes spread across the various structural components of the breast justifies its inclusion in the Fibroepithelial category of breast diseases. In the index study, FCDs are the second most common BBD constituting 30.21% of cases. This figure approximates what was reported in a previous from our center as well as reported figures from Kano and Benin. In all these cited Nigerian centers and in other African reports,, it ranked as the second most common BBD. However, the dominant patterns reported from Caucasian and Asian populations showed it to be the most common BBD.
The age range and mean age for FCDs in this study were 31–40 years and 33.6 years, respectively. Comparable mean ages of 30 and 39.5 years were reported in Nigeria from Benin and Ibadan centers, respectively. Furthermore, as opposed to FA, our study showed that cases of FCDs continued to show relatively high frequency through the fourth and into the fifth decades of life. This supports the general notion that FCD affects older age groups as compared to FA.,
Six cases of FCDs (1.8% of BBDs) were high-grade lesions (ADH). Forae et al. and Olu-Eddo et al. reported 3% and 2.7%, respectively, as proportions of such high-grade FCD lesions. A common trend that we noticed in the course of our current work is that many pathologists do not grade FCD lesions, thereby creating paucity in literature about the relative proportion of the various grades ultimately limiting proper comparison.
Benign phyllodes tumors constituted 1.1% of the BBD cases. The World Health Organization book on breast tumors reported it as constituting <1% of all breast tumors globally. However, local reports from many centers have consistently reported a higher frequency for phyllodes tumor than the global average.,,, Our finding therefore is in synchrony with reported local patterns.
Inflammatory lesions accounted for 9.4% of BBDs. Previously recorded figures in local literature include 8.1%, 4.6%, and 6.0%. Exact documentation of the frequency of inflammatory lesions of the breast from tissue biopsy specimen is problematic in that majority of clinical cases are been treated with Incision and Drainage of Fine Needle Aspiration followed by antibiotic use. Hence, biopsy may not be sent for histology except in suspicious cases. Of the 43 inflammatory cases seen, most were Chronic Nonspecific Mastitis and Fat Necrosis, while 10 cases of Granulomatous Mastitis were recorded. Collaboration with microbiology department for AFB and fungal identification and (or) culture is desirable.
The purely stromal breast lesions seen were Fibrous Change, Pseudoaangiomatous Stromal Hyperplasia, Fibrolipoma, and Granular Cell Tumor. They all together constituted 3.9% of all BBD. Previous reports,, have consistently shown this BBD subgroup as not very common in our environment.
Purely epithelial benign lesions of the breast seen in the index study were 12 cases of Tubular Adenoma, and six cases of intraductal papilloma. They constituted 2.64% and 1.32% of BBDs, respectively. This is in keeping with findings of a previous report from our center and with that of other works across Nigeria.,
All benign lesions of the male breast seen in our study were cases of gynecomastia and most of the patients were above 50 years of age. The frequency and age distribution were consistent reported local patterns.,,
| Conclusion|| |
BBDs are universally common particularly among women. They are the most common breast disease in Zaria and constituted 71.9% of all breast diseases diagnosed histologically. The peak incidence of this group of diseases is in the third decade of life. FA and FCDs were the most BBDs.
A relatively low prevalence of premalignant lesions, not reflective of the high incidence of breast cancer in our environment was also observed. Routine mammographic screening aimed at early detection of these lesions is therefore highly recommended. Equally, adequate awareness campaign aimed at highlighting the potential malignant transformation in some of these lesions will go a long way in aiding early screening, detection, and follow-up which will help toward lessening the burden of these diseases in our environment.
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Conflicts of interest
There are no conflicts of interest.
| References|| |
Murillo Ortiz B, Botello Hernández D, Ramírez Mateos C, Reynaga García FJ. Benign breast diseases: Clinical, radiological and pathological correlation. Ginecol Obstet Mex 2002;70:613-8.
Pollitt J, Gateley CA. Management of benign breast diseases of the breast. Surgery 2004;66:164-8.
Olu-Eddo AN, Ugiagbe EE. Benign breast diseases in an African population: A 25-year histopathological review of 1864 cases. Niger Med J 2011;52:211-6. [Full text]
Yusuf LM, Odigie VI, Mohammed A. Breast masses in Zaria, Nigeria. Ann Afr Med 2003;2:13-6.
Imam MI, Yawale I, Aminu ZM. Histopathological review of breast tumours in Kano, Nothern Nigeria. Sub Saharan Afr J Med 2015;2:47-51.
Otu AA. Benign breast tumours in an African population in South East. JR Coll Surg Edinb 1990;35:373-5.
Okobia MN, Osime UA. A clinicopathological study of benign breast diseases in Benin City, Nigeria. Niger J Surg 1998;5:64-8.
Adeniji KA, Adelusola KA, Odesanmi WO. Benign disease of the breast in Ile-Ife: A 10 year experience and literature review. Cent Afr J Med 1997;43:140-3.
Mayun AA, Pindiga UH, Babayo UD. Pattern of histopathological diagnosis of breast lesions in Gombe, Nigeria. Niger J Med 2008;17:159-62.
Bjerregaard B, Kung'u A. Bening breast lesions in Kenya: A histological study. East Afr Med J 1992;69:231-5.
McFarlane ME. Benign breast diseases in an Afro-Caribbean population. East Afr Med J 2001;78:358-9.
Santen RJ. Benign breast disease in women. In: Feingold KR, Anawalt B, Boyce A, Chrousus G, de herder WW, Dhatariya K, et al
., editors. Endotext. South Dartmouth (MA): MDText.com, Inc.; 2000. Available from: https://www.ncbi.nlm.nih.gov/books/NBK278994/
. [Last updated on 2018 May 25].
Ngadda HA, Gali BM, Bakari AA, Yawe-Terna EH, Tahir MB, Apari E, et al.
The spectrum of female breast diseases among Nigerian population in Sahel climatic zone. J Med Med Sci 2011;2:1157-61.
Bewtra C. Fibroadenoma in women in Ghana. Pan Afr Med J 2009;2:11.
Tavassoli FA, Devilee P. Pathology and genetics of tumours of the breast and female genital organs. In: World Health Organization Classification of Breast Tumours. Lyon (France): IARC Press; 2005. p. 9-110.
John G, Laura L, Jesse M, Jeffrey M. Rosai and Ackerman's Surgical Pathology. 10th
ed. Philadelphia: Elsevier; 2017.
Echejoh G, Dzuachi D, Jenrola A. Histopathologic analysis of benign breast diseases in Makurdi, North Central Nigeria. Int J Med Med Sci 2011;3:125-8.
Ochicha O, Edino ST, Mohammed AZ, Umar AB, Atanda AT. Benign breast lesions in Kano. Niger J Surg Res 2002;4:1-5.
Ajayi OO, Adekunle O. Non-malignant breast masses in an African population. Br J Surg 1973;60:465-8.
Aisha M, Shahida AK, Arshad MM. Changing patterns of benign breast lumps in young females. World J Med Sci 2007;2:21-4.
Nwafor CC, Udo IA. Histological characteristics of breast lesions in Uyo, Nigeria. Niger J Surg 2018;24:76-81.
] [Full text]
Stanley CU, Ezenwa PU. Benign breast lesions in Bayelsa, Niger Delta Nigeria: A 5 year multicenter histopathological audit. Pan Afr Med J 2014;19:394.
Hartmann LC, Sellers TA, Frost MH, Lingle WL, Degnim AC, Ghosh K, et al.
Benign breast disease and the risk of breast cancer. N Engl J Med 2005;353:229-37.
Cheng J, Qiu S, Raju U, Wolman SR, Worsham MJ. Benign breast disease heterogeneity: Association with histopathology, age, and ethnicity. Breast Cancer Res Treat 2008;111:289-96.
Irabor DO, Okolo CA. An audit of 149 consecutive breast biopsies in Ibadan, Nigeria. Pak J Med Sci 2008;24:257-62.
Forae GD, Nwachokor FN, Igbe AP, Odokuma EI, Ijomone EA. Benign breast diseases in Warri Southern Nigeria: A spectrum of histopathological analysis. Ann Nigeria Med 2014;8:28-31.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5]