|Year : 2019 | Volume
| Issue : 2 | Page : 126-131
Antimicrobial resistance pattern of enterococci isolated from stool samples in a tertiary hospital in Nigeria
Shuwaram Amina Shettima1, Kenneth Chukwuemeka Iregbu2
1 Department of Medical Microbiology, Parasitology and Immunology, Federal Medical Centre, Yola, Nigeria
2 Department of Medical Microbiology and Parasitology, National Hospital, Abuja, Nigeria
|Date of Submission||03-Jan-2019|
|Date of Decision||24-Mar-2019|
|Date of Acceptance||02-Apr-2019|
|Date of Web Publication||6-Dec-2019|
Dr. Shuwaram Amina Shettima
Department of Medical Microbiology, Parasitology and Immunology, Federal Medical Centre, Yola, P.M.B. 2017, Adamawa
Source of Support: None, Conflict of Interest: None
Background: Enterococci cause infections both in and out of the hospital setting and have demonstrated resistance to almost all classes of drugs. A combination of cell wall acting agents and high-level aminoglycosides is a commonly used regimen for serious infections, but resistance to either renders the synergism ineffective. Vancomycin is the drug of choice for life-threatening infections, but there have been increasing reports of resistance to the drug. Vancomycin-resistant enterococci (VRE) infection is usually preceded by gastrointestinal colonization. Aim: This study was carried out to determine the antimicrobial resistance profile of Enterococcus species isolated from stool and the prevalence of VRE. Materials and Methods: Enterococci were identified from stool samples based on characteristic growth patterns on Bile Esculin Agar and MacConkey agar and growth in 6.5% sodium chloride broth. Speciation was by conventional biochemical identification. Antibiotic susceptibility testing and screening for high-level aminoglycoside resistance (HLAR) were done by modified Kirby–Bauer disk diffusion technique. Susceptibility of isolates to linezolid, penicillin, nitrofurantoin, high-level gentamicin and streptomycin, tetracycline, ciprofloxacin, vancomycin, and teicoplanin was tested. VRE screening was done using a chromogenic agar. The polymerase chain reaction was used for confirmation. Results: Nine species of Enterococcus were identified from 561 isolates. The most common species were Enterococcus faecium (46.0%), Enterococcus faecalis (21.6%), Enterococcus gallinarum (18.5%), and Enterococcus casseliflavus (5.2%). Resistance was highest to ciprofloxacin, tetracycline, and nitrofurantoin. Lowest resistance was to vancomycin, teicoplanin, gentamicin, and linezolid. VRE prevalence rate was 1.1% and that of HLAR was 20.7%. All VRE had vanA gene. Conclusion: Overall, E. faecium was the predominant species. Highest resistance was to ciprofloxacin and tetracycline.
Keywords: Enterococcus, high-level aminoglycoside resistance, vancomycin-resistant enterococci
|How to cite this article:|
Shettima SA, Iregbu KC. Antimicrobial resistance pattern of enterococci isolated from stool samples in a tertiary hospital in Nigeria. Ann Trop Pathol 2019;10:126-31
|How to cite this URL:|
Shettima SA, Iregbu KC. Antimicrobial resistance pattern of enterococci isolated from stool samples in a tertiary hospital in Nigeria. Ann Trop Pathol [serial online] 2019 [cited 2021 Sep 26];10:126-31. Available from: https://www.atpjournal.org/text.asp?2019/10/2/126/272418
| Introduction|| |
Enterococci are multidrug-resistant opportunistic pathogens that have been implicated in serious and life-threatening healthcare-associated infections such as catheter-related urinary tract infection (UTI), intra-abdominal and pelvic infections, surgical site infections, and bacteremia.,,, Due to their remarkable ability to adapt to the environment, they acquire antibiotic resistance determinants either by a mutation in deoxyribonucleic acid (DNA) or by acquisition of new DNA through plasmids or transposons.,,
Treatment is often challenging and depends on the species, resistance patterns, location, and severity of infection. Gastrointestinal colonization often precedes infection; hence, species identification and knowledge of antibiotic resistance profile of gastrointestinal commensals are essential for formulation of guidelines for empiric and targeted therapy., In addition, targeted surveillance for identification of vancomycin-resistant enterococci (VRE) is necessary for institution of proper infection control measures to avoid dissemination of resistant strains. Early detection of VRE through highly sensitive screening methods is also important for preventing the emergence of vancomycin-resistant Staphylococcus aureus.
Since colonized patients are the primary reservoir of VRE, the use of stool sample for screening will give a better reflection of the prevalence, because rectal swab samples have been associated with a high false-negative rate.
| Materials and Methods|| |
A cross-sectional study conducted over 2 years (August 2013–August 2015). Enterococci were isolated from stool samples of adult patients (18 years and above) after written informed consent was obtained. Stool samples were self-collected in clean, dry, and leak-proof plastic containers. They were transported to the laboratory immediately, kept at room temperature, and processed as soon as possible. They were identified based on the observation of tiny brown/black colonies on Bile Esculin Agar and tiny magenta-colored colonies on MacConkey agar. Further identification was done by Gram staining and growth in 6.5% sodium chloride broth (Oxoid, Basingstoke, UK).
Isolates were inoculated onto nutrient agar slants and stored as working cultures at 4°C–8°C for subsequent antibiotic susceptibility testing and chromogenic VRE screening.
Speciation was conducted using conventional biochemical methods based mainly on carbohydrate fermentation reactions using 1% solution of (sucrose, sorbose, mannitol, sorbitol, raffinose, and arabinose) as described by Facklam and Collins.
Antibiotic susceptibility testing of all confirmed Enterococcus isolates was performed and interpreted according to the recommendations of the Clinical and Laboratory Standards Institute, using the modified Kirby–Bauer disk diffusion method, on Mueller–Hinton agar (Oxoid, Basingstoke, UK) for all the antibiotics tested except glycopeptides (vancomycin and teicoplanin) which were tested using the epsilometer test (E-test) method.
Antibiotics tested were β-lactam (penicillin – 10 units), quinolone (ciprofloxacin – 5 μg), aminoglycosides (gentamicin – 120 μg and streptomycin – 300 μg), tetracyclines (tetracycline – 30 μg), nitrofurantoins (nitrofurantoin – 300 μg), and oxazolidinones (linezolid – 30 μg). Susceptibility of each isolate to vancomycin and teicoplanin was tested using E-test strips for minimum inhibitory concentration (MIC) determination (Liofilchem, Roseto Degli Abruzzi, Italy). Plates were incubated for a full 24 h for accurate detection of resistance. Enterococcus faecalis ATCC® 29212 was used as the quality control strain.
High-level aminoglycoside resistance (HLAR) screening was done using disk diffusion method (high content gentamicin –120 μg and streptomycin – 300 μg disks)., Resistance was indicated by absence of zone of inhibition and susceptibility by a zone diameter of ≥10 mm for both gentamicin and streptomycin [Figure 1].
|Figure 1: Vancomycin epsilometer test strip on Mueller–Hinton agar showing sensitivity with minimum inhibitory concentration of 1.0 μg/ml. High content gentamicin and streptomycin antibiotic disks showing zone of inhibition|
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In this study, HLAR is defined as resistance to high-level gentamicin alone (HLGR), or high-level streptomycin alone (HLSR) or resistance to both.
E. faecalis ATCC® 29212 was used as the quality control strain (sensitive).
Chromogenic screening for vancomycin resistance was carried out on all confirmed enterococcal isolates using a chromogenic screening agar, CHROMagar™ VRE (CHROMagar Co, Paris, France). Pure colonies on nutrient agar were inoculated onto CHROMagar VRE and incubated for 24–48 h at 37°C in ambient air. Pink colonies were presumptively identified as VRE. faecalis/VR E. faecium [Figure 2], while blue colonies were identified as VR E. gallinarum/VR E. casseliflavus according to manufacturer's specifications. Agar plates showing no growth or growth with other colors were regarded as negative for VRE.
|Figure 2: Vancomycin-resistant enterococci colonies on CHROMagar plate. Vancomycin-resistant enterococci are revealed in pink color (Enterococcus faecium)|
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Presumptively identified VRE were inoculated into the nutrient broth and stored at −20°C for subsequent molecular analysis by polymerase chain reaction (PCR) for vanA and vanB genes detection. The purified DNA of all presumptive VRE isolates was obtained using the phenol-chloroform DNA extraction method. The specific vancomycin-resistant genotype (vanA, vanB) was determined by genomic DNA amplification with real-time PCR analysis using specific primers (Bio-Rad Laboratories Inc., Marnes-la-Coquette, France) selected from published gene sequences.
The extracted DNA and the forward and reverse primers for the resistance genes were added to a PCR master mix, GreenStar™ qPCR PreMix (Bioneer; Alameda, CA, US) for each test according to the manufacturer's instructions.
PCR amplifications were done in 1.5 mL reaction tubes each with reaction mixtures composed of 6.5μl of sample DNA (extracted enterococcal genomic DNA), 0.5μl (20 pmol) of each primer and 12.5μl of GreenStar™ qPCR PreMix were prepared and aliquoted in 20μl quantities in individual PCR capillary tubes.
Real-time PCR was used for amplification of fragments representing vanA/vanB genes, using Roche LightCycler, a quantitative real-time PCR thermal cycler (Roche Life Science, Mannheim, Germany).
The thermal cycling conditions were initial denaturation at 95°C for 10 min, then 40 cycles of denaturation at 95°C for 15 s, annealing (50°C–52°C), depending on the primer pairs, for 15 s and extension at 72°C for 30 s.
Enterococcus faecium ATCC® 51559 was used as vanA-positive control strain. E. faecalis ATCC® 51299 was vanB-positive control. Negative control for each test consisted of PCR reagent master mix and 6.5μl of sterile molecular grade water.
A real-time PCR standard curve was generated for each test using the LightCycler software version 3.5 (Roche Life Science, Mannheim, Germany).
DNA extraction and PCR were done at DNA laboratories, a diagnostic and research laboratory with facilities for molecular biology located at Kaduna, Nigeria.
Statistical analysis of data was performed using statistical package for social sciences (SPSS) software version 20 (IBM Corp., Armonk, NY, United States).
Ethical approval for the study was obtained from the institution's Health Research Ethics Committee.
| Results|| |
Five-hundred and sixty-one enterococci were isolated from stool samples. Of these, 258 (46.0%) were identified as E. faecium, 121 (21.6%) were E. faecalis, 104 (18.5%) were Enterococcus gallinarum, and 29 (5.2%) were Enterococcus casseliflavus. The remaining 8.7% comprised Enterococcus hirae, Enterococcus durans, Enterococcus mundtii, Enterococcus raffinosus, Enterococcus dispar, and unidentified species.
Antimicrobial resistance of enterococci
Of the 561 isolates, 92 (16.4%) were resistant to linezolid, 149 (26.6%) resistant to penicillin, 241 (43.0%) to nitrofurantoin, 367 (65.4%) to tetracycline, and 399 (71.1%) to ciprofloxacin [Table 1].
|Table 1: Antimicrobial resistance profile of Enterococcus isolates (disk diffusion method)|
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A total of 116 of 561 isolates (20.7%) were HLAR. Of these 116 HLAR isolates, 4 (3.4%) were resistant to high-level gentamicin alone, 74 (63.8%) were resistant to high-level streptomycin alone, and 38 (32.8%) demonstrated resistance to both. Forty-two isolates in total (7.5%) had high-level gentamicin resistance (HLGR), while a total of 112 (20.0%) were high-level streptomycin resistant (HLSR) (P 0.02) [Table 1].
Linezolid resistance was observed in 43 (16.7%) E. faecium, 15 (12.4%) E. faecalis, 16 (15.4%) E. gallinarum, and 9 (31.0%) of E. casseliflavus [Table 2].
|Table 2: Antimicrobial resistance pattern of four most common Enterococcus species isolated in the study|
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With the E-test method, all isolates in the study were sensitive to both vancomycin and teicoplanin (MIC for both glycopeptides ranged between 0.38 and 4.0 μg/mL) [Figure 1], with the exception of one E. gallinarum isolate which was intermediately sensitive to vancomycin (MIC of 8.0 μg/mL).
However, chromogenic agar screening identified 6 (1.1%) presumptive VRE (including the E. gallinarum which showed intermediate sensitivity with the E-test method). All the six presumptively identified VRE (100.0%) had vanA genotype detected by real-time PCR, while none had vanB genotype.
Of the six VRE, 3 (50.0%) were E. faecium, 2 (33.3%) E. gallinarum, and 1 (16.7%) E. casseliflavus.
Vancomycin-resistant enterococci antibiotic resistance pattern
All (100.0%) the VRE were resistant to linezolid, tetracycline, and ciprofloxacin; 4 (66.7%) to penicillin, 3 (50.0%) to nitrofurantoin, 1 (16.7%) to high-level gentamicin, and 1 (16.7%) to high-level streptomycin. All the E. faecium were resistant to penicillin and nitrofurantoin.
Three isolates, one E. durans, one E. casseliflavus, and one E. raffinosus, were resistant to all classes of antibiotics tested except glycopeptides (vancomycin and teicoplanin).
| Discussion|| |
E. faecium and E. faecalis were the two most commonly isolated enterococcal species from stool samples in our study. Worldwide, the same pattern is seen both from clinical samples and from gastrointestinal commensals, although other species are increasingly being isolated as well.,,,,,
Although the high linezolid sensitivity seen in this study is similar to findings elsewhere across the world,,,, the resistance level observed is worrisome, considering that this drug is rarely used in our environment and mechanisms of resistance to other ribosomal protein synthesis inhibitors do not confer cross resistance to it. Previous studies have similarly reported linezolid resistance in the absence of selective drug pressure., This was attributed to horizontal transfer of plasmid-mediated resistance to linezolid due to cfr gene, which encodes a 23S rRNA methyltransferase., The same mechanism may apply in our environment as linezolid resistance may have been imported by many of our patients who go on medical tourism to other countries where clinical use of the drug is more common.
E. casseliflavus demonstrated the highest resistance to linezolid of the four most common species isolated. Although there have been reports of linezolid-resistant E. casseliflavus from both human and animal origin, high rates of resistance to this drug among this species have rarely been reported.,
Resistance of majority of the isolates to ciprofloxacin is similar to results obtained from previous studies reported by other authors.,, This high rate of resistance is likely due to selective drug pressure from intense use in the hospital and the community. In the hospital setting, fluoroquinolones such as ciprofloxacin are a common choice for empiric treatment of UTI, of which enterococci are a common cause.,, A study in Japan demonstrated that fluoroquinolone resistance was significantly associated with previous use of fluoroquinolones, which in turn was significantly related with amino acid mutations in the quinolone resistance-determining regions which ultimately results in resistance.
Overall, sensitivity to glycopeptides, high-level aminoglycosides, and linezolid is high. This finding is reassuring because it is an indication that these agents can be used as empiric therapy for life-threatening enterococcal infections in our environment.
The relatively high prevalence of HLAR, in this study, agrees with findings of a study conducted in Bangladesh. The higher level of resistance of enterococci to streptomycin compared to gentamicin is similar to the pattern observed in previous studies conducted in Nigeria and Iran., The higher rate of streptomycin resistance may be explained by the fact that enterococci can develop resistance to this agent through multiple mechanisms which include enzymatic mechanisms associated with production of aminoglycoside-modifying enzymes and high-level resistance to streptomycin arising from just a single-step mutation in the 30S ribosomal subunit.,
The finding of three commensal isolates (E. durans, E. casseliflavus, and E. raffinosus) with resistance to all antibiotics, except glycopeptides, is not surprising as enterococci in the guts of humans and animals easily acquire resistance genes from other gut flora under selective pressure from ongoing use of antimicrobials.,
CHROMagar VRE demonstrated 100% sensitivity in detecting VRE, and this finding agrees with the reports from previously conducted studies on chromogenic screening for VRE.,, The PCR method also identified the vanA gene in the same isolates, while the E-test method did not detect any VRE. It is a known fact that diffusion methods are usually unreliable for detection of vancomycin resistance, especially in cases of low-level inducible resistance, and this may be attributed to the large size of the vancomycin molecule which has difficulty diffusing through agar media.
The high concordance between CHROMagar VRE and PCR in the detection of VRE is of clinical interest because an accurate, rapid, and cost-effective screening method are desirable for prompt institution of treatment and control measures. The implication of the finding in this study is that CHROMagar VRE can be reliably used to screen and detect VRE in the absence of expensive molecular systems in low- and middle-income countries such as Nigeria.
In this study, only vanA gene was found, similar to the finding of Akpaka et al. in Bermuda.vanA gene has been reported to be the most common vancomycin resistance gene.,
E. faecium is known to be a major reservoir of acquired vancomycin resistance, and this was also seen in our work as it constituted majority of the VRE. The low prevalence of VRE in this study, when compared to reports from the United States as well as countries in Asia,,, may be a reflection of the low use of vancomycin in this locality. A previous study in Lagos, Nigeria, by Iregbu et al. found no VRE among enterococci isolates.
The relatively lower resistance of VRE to the high-level aminoglycosides compared to the other antibiotics tested means that these agents could still be used for the treatment of VRE infections in our environment in combination with a cell wall active agent if the isolate is susceptible.
The 100% resistance of VRE to linezolid in our study is worrisome. This is in view of the fact that it is one of the few drugs used for management of VRE infections. Similar finding of linezolid resistance among VRE has been reported from different regions.,,
| Conclusion|| |
Isolates colonizing the gastrointestinal tract of patients seen in our study were sensitive to glycopeptides, linezolid, and high-level aminoglycosides. These drugs should be used for empiric treatment of enterococcal infections in our environment. Although vancomycin resistance was very low and limited to a few species, there is need for surveillance among hospitalized patients using rapid and accurate techniques like chromogenic agar screening to control the spread of VRE.
National Hospital, Abuja provided financial assistance.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Arias CA, Murray BE. Enterococcus
Species, Streptococcus bovis
Group, and Leuconostoc
Species. In: Mandell GL, Bennett JE, Dolin R, editors. Principles and Practice of Infectious Diseases. 7th
ed. Philadelphia, PA: Churchill Livingstone, Elsevier; 2010. p. 2643-53.
Shokoohizadeh L, Ekrami A, Labibzadeh M, Ali L, Alavi SM. Antimicrobial resistance patterns and virulence factors of enterococci isolates in hospitalized burn patients. BMC Res Notes 2018;11:1.
Iregbu KC, Ogunsola FT, Odugbemi TO. Susceptibility profile of Enterococcus faecalis
isolated at the Lagos University teaching hospital, Nigeria. Niger Postgrad Med J 2002;9:125-8.
Khodabandeh M, Mohammadi M, Abdolsalehi MR, Hasannejad-Bibalan M, Gholami M, Alvandimanesh A, et al.
High-level aminoglycoside resistance in Enterococcus faecalis
and Enterococcus faecium
; as a serious threat in hospitals. Infect Disord Drug Targets 2018. [E-pub ahead of print].
Ahmed MO, Baptiste KE. Vancomycin-resistant enterococci: A Review of antimicrobial resistance mechanisms and perspectives of human and animal health. Microb Drug Resist 2018;24:590-606.
Daniel DS, Lee SM, Dykes GA, Rahman S. Public health risks of multiple-drug-resistant Enterococcus
spp. In Southeast Asia. Appl Environ Microbiol 2015;81:6090-7.
Miller WR, Munita JM, Arias CA. Mechanisms of antibiotic resistance in enterococci. Expert Rev Anti Infect Ther 2014;12:1221-36.
Taimur S, Miller NS, Whitney D, Barlam T. Empiric and targeted treatment of enterococcal infections: Opportunities for antimicrobial stewardship. Infect Dis Clin Pract 2015;23:72-5.
Abamecha A, Wondafrash B, Abdissa A. Antimicrobial resistance profile of Enterococcus
species isolated from intestinal tracts of hospitalized patients in Jimma, Ethiopia. BMC Res Notes 2015;8:213.
Akhter S, Asna ZH, Rahman MM. Prevalence and antimicrobial susceptibility of Enterococcus
species isolated from clinical specimens. Mymensingh Med J 2011;20:694-9.
Salem-Bekhit MM, Moussa IM, Muharram MM, Alanazy FK, Hefni HM. Prevalence and antimicrobial resistance pattern of multidrug-resistant enterococci isolated from clinical specimens. Indian J Med Microbiol 2012;30:44-51.
] [Full text]
Siegel JD, Rhinehart E, Jackson M, Chiarello L. Healthcare infection control practices advisory committee. Management of multidrug-resistant organisms in health care settings, 2006. Am J Infect Control 2007; 35(10 Suppl 2):S165-93.
de Niederhäusern S, Bondi M, Messi P, Iseppi R, Sabia C, Manicardi G, et al.
Vancomycin-resistance transferability from VanA enterococci to Staphylococcus aureus
. Curr Microbiol 2011;62:1363-7.
Chen AY, Zervos MJ. Enterococcus
: Antimicrobial resistance in enterococci; epidemiology, treatment and control. In: Mayers DL, Lerner SA, Ouellette M, Sobel JD, editors. Antimicrobial Drug Resistance; Clinical and Epidemiological Aspects. New York: Springer; 2009. p. 715-33.
DAgata EM, Gautam S, Green WK, Tang YW. High rate of false-negative results of the rectal swab culture method in detection of gastrointestinal colonization with vancomycin-resistant enterococci. Clin Infect Dis 2002;34:167-72.
Facklam RR, Collins MD. Identification of Enterococcus
species isolated from human infections by a conventional test scheme. J Clin Microbiol 1989;27:731-4.
Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-second Informational Supplement. Wayne, PA: Clinical and Laboratory Standards Institute M100-S22; 2012. p. 90-4.
Washington W, Stephen A, William J, Elmer K, Gary P, Paul S, et al
. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 6th
ed. Philadelphia, USA: Lippincott Williams and Wilkins; 2006. p. 672-764.
Hajia M, Rahbar M, Zadeh MM. A novel method “CHROMagar” for screening vancomycin-resistant enterococci (VRE) isolates. Afr J Biotechnol 2012;11:9865-8.
Honarm H, Falah Ghavidel M, Nikokar I, Rahbar Taromsari M. Evaluation of a PCR assay to detect Enterococcus Faecalis
in blood and determine glycopeptides resistance genes: Van a and van B. Iran J Med Sci 2012;37:194-9.
Ghasemi E, Mansouri S, Shahabinejad N. Vaginal colonization and susceptibility to antibiotics of enterococci during late pregnancy in Kerman City, Iran. Arch Clin Infect Dis 2016;11:e35428.
Coombs GW, Pearson JC, Daley DA, Le T, Robinson OJ, Gottlieb T, et al.
Molecular epidemiology of enterococcal bacteremia in Australia. J Clin Microbiol 2014;52:897-905.
Parameswarappa J, Basavaraj VP, Basavaraj CM. Isolation, identification, and antibiogram of enterococci isolated from patients with urinary tract infection. Ann Afr Med 2013;12:176-81.
] [Full text]
Zhang Y, Du M, Chang Y, Chen LA, Zhang Q. Incidence, clinical characteristics, and outcomes of nosocomial Enterococcus
spp. Bloodstream infections in a tertiary-care hospital in Beijing, China: A four-year retrospective study. Antimicrob Resist Infect Control 2017;6:73.
Aamodt H, Mohn SC, Maselle S, Manji KP, Willems R, Jureen R, et al.
Genetic relatedness and risk factor analysis of ampicillin-resistant and high-level gentamicin-resistant enterococci causing bloodstream infections in Tanzanian children. BMC Infect Dis 2015;15:107.
Ekuma AE, Oduyebo OO, Efunshile AM, Konig B. Surveillance for vancomycin resistant enterococci in a tertiary institution in South Western Nigeria. Afr J Infect Dis 2016;10:121-6.
Akpaka PE, Kissoon S, Wilson C, Jayaratne P, Smith A, Golding GR. Molecular characterization of vancomycin-resistant Enterococcus faecium
isolates from Bermuda. PLoS One 2017;12:e0171317.
Eliopoulos GM, Meka VG, Gold HS. Antimicrobial resistance to linezolid. Clin Infect Dis 2004;39:1010-5.
Arias CA, Vallejo M, Reyes J, Panesso D, Moreno J, Castañeda E, et al.
Clinical and microbiological aspects of linezolid resistance mediated by the cfr gene encoding a 23S rRNA methyltransferase. J Clin Microbiol 2008;46:892-6.
Diaz L, Kiratisin P, Mendes RE, Panesso D, Singh KV, Arias CA. Transferable plasmid-mediated resistance to linezolid due to cfr in a human clinical isolate of Enterococcus faecalis
. Antimicrob Agents Chemother 2012;56:3917-22.
Chatzigeorgiou KS, Tarpatzi A, Siafakas N, Pantelaki M, Charalampaki N, Zerva L. Identification and Susceptibility Testing of Enterococcus casseliflavus
and Enterococcus gallinarum
by Phoenix 100 System (Becton Dickinson). 18th
European Congress on Clinical Microbiology and Infectious Disease. Barcelona, Spain: European Society of Clinical Microbiology and Infectious Diseases; 2008.
Liu Y, Wang Y, Dai L, Wu C, Shen J. First report of multiresistance gene cfr in Enterococcus
of swine origin. Vet Microbiol 2014;170:352-7.
Golia S, Nirmala AR, Kamath BA. Isolation and speciation of enterococci from various clinical samples and their antimicrobial susceptibility pattern with special reference to high level aminoglycoside resistance. Int J Med Res Health Sci 2014;3:526-9.
Jafari-Sales A, Sayyahi J, Akbari-Layeg F, Mizabi-Asl M, Rasi-Bonab F, Abdoli-senejani M, et al
. Identification of gyrA Gene in ciprofloxacin-resistant Enterococcus faecalis
in strains isolated from clinical specimens in hospitals and clinics of Tabriz and Marand Cities. Arch Clin Microbiol 2017;8:63.
Sinel C, Cacaci M, Meignen P, Guérin F, Davies BW, Sanguinetti M, et al.
Subinhibitory concentrations of ciprofloxacin enhance antimicrobial resistance and pathogenicity of Enterococcus faecium.
Antimicrob Agents Chemother 2017;61. pii: e02763-16.
Iregbu KC, Nwajiobi-Princewill PI. Urinary tract infections in a tertiary hospital in Abuja, Nigeria. Afr J Clin Exp Microbiol 2013;14:169-73.
Yasufuku T, Shigemura K, Shirakawa T, Matsumoto M, Nakano Y, Tanaka K, et al.
Mechanisms of and risk factors for fluoroquinolone resistance in clinical Enterococcus faecalis
isolates from patients with urinary tract infections. J Clin Microbiol 2011;49:3912-6.
Dubin K, Pamer EG. Enterococci and their interactions with the intestinal microbiome. Microbiol Spectr 2014;5.
Wurster JI, Saavedra JT, Gilmore MS. Impact of antibiotic use on the evolution of Enterococcus faecium
. J Infect Dis 2016;213:1862-5.
Kim DH, Lee JH, Ha JS, Ryoo NH, Jeon DS, Kim JR. Evaluation of the usefulness of selective chromogenic agar medium (chromID VRE) and multiplex PCR method for the detection of Vancomycin-resistantenterococci. Korean J Lab Med 2010;30:631-6.
Ledeboer NA, Das K, Eveland M, Roger-Dalbert C, Mailler S, Chatellier S, et al.
Evaluation of a novel chromogenic agar medium for isolation and differentiation of vancomycin-resistant Enterococcus faecium
and Enterococcus faecalis
isolates. J Clin Microbiol 2007;45:1556-60.
Pendle S, Jelfs P, Olma T, Su Y, Gilroy N, Gilbert GL. Difficulties in detection and identification of Enterococcus faecium
with low-level inducible resistance to vancomycin, during a hospital outbreak. Clin Microbiol Infect 2008;14:853-7.
Wanger A. Disk diffusion test and gradient methodologies. In: Schwalbe R, Steele-Moore L, Goodwin AC, editors. Antimicrobial Susceptibility Testing Protocols. New York, USA: CRC Press; 2007. p. 53-72.
d'Azevedo PA, Santiago KA, Furtado GH, Xavier DB, Pignatari AC, Titze-de-Almeida R. Rapid detection of vancomycin-resistant enterococci (VRE) in rectal samples from patients admitted to intensive care units. Braz J Infect Dis 2009;13:289-93.
Kramer TS, Remschmidt C, Werner S, Behnke M, Schwab F, Werner G, et al.
The importance of adjusting for Enterococcus
species when assessing the burden of vancomycin resistance: A cohort study including over 1000 cases of enterococcal bloodstream infections. Antimicrob Resist Infect Control 2018;7:133.
Monteserin N, Larson E. Temporal trends and risk factors for healthcare-associated vancomycin-resistant enterococci in adults. J Hosp Infect 2016;94:236-41.
Alotaibi FE, Bukhari EE. Emergence of vancomycin-resistant enterococci at a teaching hospital, Saudi Arabia. Chin Med J (Engl) 2017;130:340-6.
Purohit G, Gaind R, Dawar R, Verma PK, Aggarwal KC, Sardana R, et al.
Characterization of vancomycin resistant enterococci in hospitalized patients and role of gut colonization. J Clin Diagn Res 2017;11:DC01-5.
O'Driscoll T, Crank CW. Vancomycin-resistant enterococcal infections: Epidemiology, clinical manifestations, and optimal management. Infect Drug Resist 2015;8:217-30.
de Almeida LM, de Araújo MR, Iwasaki MF, Sacramento AG, Rocha D, da Silva LP, et al.
Linezolid resistance in vancomycin-resistant Enterococcus faecalis
and Enterococcus faecium
isolates in a Brazilian hospital. Antimicrob Agents Chemother 2014;58:2993-4.
Krull M, Klare I, Ross B, Trenschel R, Beelen DW, Todt D, et al.
Emergence of linezolid-and vancomycin-resistant Enterococcus faecium
in a department for hematologic stem cell transplantation. Antimicrob Resist Infect Control 2016;5:31.
Yadav G, Thakuria B, Madan M, Agwan V, Pandey A. Linezolid and vancomycin resistant enterococci: A therapeutic problem. J Clin Diagn Res 2017;11:GC07-11.
[Figure 1], [Figure 2]
[Table 1], [Table 2]