|Year : 2019 | Volume
| Issue : 1 | Page : 20-26
Molecular subtyping of carcinoma of the female breast in a tertiary teaching hospital in Northern Nigeria
Asma'u Usman1, Yawale Iliyasu2, Akinfenwa Taoheed Atanda3
1 Department of Pathology, Federal Medical Centre, Katsina, Nigeria
2 Department of Pathology, Ahmadu Bello University Teaching Hospital, Zaria, Kaduna State, Nigeria
3 Department of Pathology, Aminu Kano Teaching Hospital, Kano, Nigeria
|Date of Web Publication||14-May-2019|
Dr. Asma'u Usman
Department of Pathology, Federal Medical Centre, Katsina
Source of Support: None, Conflict of Interest: None
Background: Cancer of the breast is globally the most common female cancer including in Nigeria. Newer treatment modalities are based on tumor immunophenotyping, thus the need to characterize these tumors among women with the disease in Northern Nigeria. Aims: This study aims to classify carcinomas of the breast diagnosed in the pathology laboratory of a teaching hospital based on their expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2/neu (HER2/neu) overexpression. Methodology: The histology slides of 478 carcinomas of the breast as well as the ER, PR, and HER2 immunohistochemistry records of 118 stained cases were retrieved and reviewed. Results: Age range of cases was from 20 to 80 years (mean = 46.9 ± 13). The most common histomorphologic entity was invasive carcinoma (NST) which accounted for 73.2% of cases. These were followed in frequency by invasive lobular carcinoma accounting for 6.8% of cases and invasive papillary carcinoma representing 6.5% of cases. Overall, 169 cases (37.6%) were Grade III tumors while Grades II and I tumors accounted for 35.1% and 27.3% of cases, respectively. Triple-negative tumors were the most common molecular subtype and comprised 46.6% of all cases. Luminal B (LUMB) was the least common and accounted for 8% of all cases. HER2 overexpression and LUMA were seen in 17.9% and 28.8% of cases, respectively. Conclusion: Carcinoma of the breast in the population studied occurs at a younger age than among Caucasians. The tumors are characterized by preponderance of invasive carcinoma (NST), high histological grade, and triple-negative phenotype.
Keywords: Breast carcinoma, estrogen, human epidermal growth factor receptor 2, progesterone, receptor
|How to cite this article:|
Usman A, Iliyasu Y, Atanda AT. Molecular subtyping of carcinoma of the female breast in a tertiary teaching hospital in Northern Nigeria. Ann Trop Pathol 2019;10:20-6
|How to cite this URL:|
Usman A, Iliyasu Y, Atanda AT. Molecular subtyping of carcinoma of the female breast in a tertiary teaching hospital in Northern Nigeria. Ann Trop Pathol [serial online] 2019 [cited 2021 Jan 27];10:20-6. Available from: https://www.atpjournal.org/text.asp?2019/10/1/20/258165
| Introduction|| |
Breast cancer is the most common cancer worldwide and the second leading cause of cancer-related deaths. It comprises 22.9% of invasive cancers in women and 16% of all female cancers globally. Mortality rates in some African countries including Nigeria, Egypt, and Ethiopia have been found to be among the highest worldwide and has been attributed to late presentation and thus much poorer survival.
Several factors have been implicated in the etiopathogenesis of breast cancer, and these include advancing age, genetics, positive family history, low residue diet, alcohol, obesity, and physical inactivity. Over the last few decades, breast cancer classification has become molecular. This is in deference to its recognized heterogeneity and inadequacy of morphological features alone to completely predict tumor behavior. The expression of hormone receptors including estrogen receptor (ER), progesterone receptor (PR), or human epidermal growth factor receptor 2/neu (HER2/neu) distinguishes classes of tumors thought to be derived from different cells of origin: HR+ (positive) and HR− (negative) tumors. Based on positivity or otherwise of these receptors, carcinomas of the breast are now classified as luminal A (LUMA), LUMB, HER2 overexpressing, and basal-like (also referred to as triple-negative breast cancers; [TNBC]). These entities have been linked to different responses to different therapeutic modalities.
This study aims to evaluate the molecular characteristics of carcinomas of the breast diagnosed in a teaching hospital in Northwestern Nigeria, hence enabling development of treatment modalities for such patients.
| Materials and Methods|| |
All cases of carcinoma of the breast and their corresponding ER, PR, and HER2/neu status recorded in the archives of Pathology Department of Aminu Kano Teaching Hospital between January 2011 and December 2015 were retrieved.
The tumors were classified in accordance with the updated 2003 edition of the WHO histological classification of breast tumors and graded using the Scarff–Bloom–Richardson (SBR) grading system. This grading system is based on three different features: tubule formation, nuclear pleomorphism, and mitotic count. Each of these features is given a Score of 1–3. The individual scores are added together to give a total score between 3 and 9 and a tumor grade is assigned based on the score: well-differentiated carcinomas (Score 3–5), moderately differentiated carcinomas (Score 6–7), and poorly differentiated carcinomas (Score 8–9).
Ensuing data was managed using IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY: IBM Corp and presented with the aid of tables and charts. Statistical significance was at P < 0.05 at 95% confidence interval.
| Results|| |
A total of 3214 malignancies were diagnosed in the histopathology department of the teaching hospital over the 5-year period (2011–2015). Five hundred and twenty-four of these were breast cancers and comprised 14.9% of all these malignancies. Carcinomas accounted for 98.9% of the breast malignancies while the remaining 1.1% were sarcomas and lymphomas. No metastatic tumor to the breast was reported.
Of the 524 cases, only 478 cases were suitable for this study excluding noncarcinomas and those with incomplete biodata. Of the 478 cases that met the inclusion criteria, immunohistochemistry was performed on only 118 (24.7%) of them.
[Table 1] depicts the age distribution of the various histomorphologic entities. Ages of affected women ranged between 20 and 80 years with mean age of 46.9 ± 13. The overall age pattern was such that from age group 20 to 29 years, the frequency rose sharply from 24 (5%) cases to 113 (23.7%) cases in the 30–39-year age range, and peaked in the 40–49-year age group which accounted for 28% of all cases [Figure 1]. From here, it dropped progressively to 7 (1.5%) cases in the 80–89 age group. With a frequency of 56.7%, women <50 years of age constituted about two-third of all cases while 5% of the women were <30 years of age.
|Figure 1: Line graph showing categorized-age distribution of carcinoma of the breast|
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The most common histomorphologic type, accounting for 73.2% of cases, was invasive carcinoma (NST), invasive lobular carcinoma with 6.8% of cases, invasive papillary carcinoma with 6.5% and medullary carcinoma with 3.8% of all cases. Six cases of ductal carcinoma in situ (DCIS) representing 1.2% of all lesions were the only noninvasive carcinomas diagnosed. The occurrence of invasive carcinoma (NST) was found to be highest in the fifth decade of life. Invasive lobular carcinoma had a wider peak age distribution of 30–49 years. Invasive papillary carcinoma and medullary carcinoma were mostly diagnosed in women aged 30–39 years. However, 67% cases of DCIS were diagnosed in women in the 30–39 age bracket [Table 1].
Histomorphologic entities were graded using the SBR grading system and the pattern is as shown in [Table 2]. Four hundred and fifty cases were available for grading. The largest proportions, 37.6%, were Grade III, with Grade II and I were 35.1% and 27.3%, respectively. Grade III malignancies were more frequent among invasive carcinoma (NST) and apocrine carcinoma. Other special types including invasive lobular carcinoma, invasive papillary carcinoma, adenoid cystic carcinoma, and secretory carcinoma among others, were more frequently Grade II [Table 2].
|Table 2: “d” Scarff-Bloom-Richardson grading of the histomorphologic types of carcinomas of the breast (n=450)|
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The various molecular subtypes and their percentage distribution are shown in [Figure 2]. In this respect, triple negative was the most frequent with 46.6% (55 of 118 cases), followed by LUMA 28.8% (34 of 118 cases), HER2 overexpressed 17.9% (21 of 118 cases), and least of all LUMB 8% (8 of 118 cases). Thus, 35.5% of the cases were ER-positive (LUMA + LUMB, [Figure 3] and [Figure 4]) while 64.5% were ER-negative (HER2 overexpression + triple negative, [Figure 5]).
|Figure 3: Photomicrograph showing strong nuclear staining in almost 100% of the invasive cells (Estrogen receptor positive; 5+3). ×10|
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|Figure 4: Photomicrograph showing strong nuclear staining in almost 100% of the cells (Progesterone receptor positive; 5+3). ×10|
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|Figure 5: “d” Photomicrograph showing Invasive carcinoma (NST) demonstrating HER2 immunostaining: (a) HER2- (×10); (b) Incomplete membrane staining HER2; 2+ (×40) and (c) Complete membrane staining HER2; 3+ (×20)|
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Overall, triple-negative subtype was the most common molecular phenotype. Between ages 30 and 49, the distribution of the TNBC was fairly uniform but rose to a peak in the sixth decade (50–59 years) and then progressively declined from the seventh decade. HER2 overexpression was most frequent in women 30–39 years of age, followed by women in the fifth and sixth decades (40–49 years and 50–59 years). LUMA tumors constituted 28.8% of all the molecular subtypes and women in the sixth decade accounted for one-third of all LUMA cases. The least common subtype was LUMB and accounted for 8% of all subtypes with an almost uniform distribution in women <60 years of age [Table 3].
[Table 4] depicts the molecular subtyping of the various histomorphologic entities. Relative to histomorphology, triple-negative subtype was still the most common, with invasive carcinoma (NST) being the most predominant histomorphologic entity in all the molecular subtypes. Half of the apocrine carcinomas (2 of 4) were triple negative.
HER2 overexpression [Figure 5] was almost exclusively seen in invasive carcinoma (NST), 15 of 21, with apocrine carcinoma accounting for 2% (1 of 26).
Only a case of mucinous carcinoma was seen and it was a LUMA tumor. The other special types were quite common but invasive carcinoma (NST) was still the most common comprising 26 of 34 for LUMA and 6 of 8 for LUMB subtypes.
| Discussion|| |
Cancer of the breast comprised 14.9% of all malignancies in Kano during the 5-year study period. This is within a national range of 11.9%–31.8%,, but is much higher than figures reported in Cote d'Ivoire (6.5%) and less than figures from Sudan (22.9%) and Iraqi (34%).,, The predominance of carcinomas (98.9%) is comparable with findings of 92.2% in Cote d'Ivoire.
The age range 20–80 years observed in this study is similar to published reports from Gombe and Ile-Ife., The mean age of 46.9 years found in the index study is in consonance with what has been reported in other parts of Nigeria as exemplified by 42.1 years reported in Zaria, 44 years in Gombe, 45.1 years in Calabar, and 48 years in Ile-Ife.,,, These findings, resonating across the country, lend credence to the observation that malignant tumors in this country generally tend to occur a decade to a decade and a half earlier than what has been described among Caucasians. Similar observation has been made among Africans and African-Americans living in the USA, and the cause of this has been postulated to reflect higher rates of BRCA gene mutation among non-Caucasians.
In further support of this is the observation that 56.7% of the women in this study were <50 years of age in addition to approximately 1 out of every 20 being younger than 30 years of age. The peak age range of 40–49 years as found in the index study is also comparable to findings in Calabar and Maiduguri in Nigeria and Iraq in the Middle East., In contrast, a bimodal peak age was described in Hong Kong with an initial peak age range of 40–45 probably reflecting a predominantly genetic influence and a second peak in the 75–85 year age range probably reflecting a predominance of environmental factors.
Invasive carcinoma (NST), like in other studies in Nigeria, was still the most common histomorphologic entity in the index study. Yet, the percentage (73.2%) was slightly lower than what was recorded in other parts of the North, 78.8% in Gombe and 82.6% in Maiduguri, as well as in the Southern part of the country with values ranging between 75.5% and 85.2%.,,, Our value is also lower than what has been reported in other parts of West Africa including Ghana and Cote d'Ivoire where it ranges between 83.2% and 92.2%, as well as outside Africa where it has been between 81% and 81.8%.,, The relatively higher occurrence of special types of the disease appears to account for this. More noteworthy, however, was the rarity of DCIS (6 of 118 cases), a finding that is in stark contrast to what is seen in the Western countries. This finding perhaps shows that late presentation is the norm in Nigeria; poor uptake of breast screening strategies, especially mammography, and as described by Odusanya andTayo  and by Uche, inadequate awareness of breast cancer among the Nigerian populace.
Grade III tumors were more common in this series of breast cancer cases with similar predominance of Grade III tumors described by most studies in Nigeria and internationally., The invasive non-NST tumors were mostly Grade II with the rest being Grade III. Other studies including that by Seshi et al. in Ghana  which reported the majority (60.8%) being Grade II tumor also contrasts with the other aforementioned studies. This disparity could be due to interobserver variability in grading as highlighted by Chowdhury et al. in their study of breast cancer grading.
ER-negative cases comprising triple-negative and HER2 overexpressed tumors were the most frequent in the index study. The 46.6% frequency of triple-negative molecular subtype found in this study is lower than the 47.7% reported by Titloye et al., the 52.6% by Minoza et al. in Maiduguri, and the 87% by Makanjuola et al. in Lagos. However, these are higher than the 6% and 16% reported by El Fatemi et al. and Adebamowo et al., respectively, succinctly underscoring the preponderance of this subtype in Nigeria. In comparison, the frequency of this subtype has been found to range between 14% and 29.5% in Western countries., The implication for Nigerian women with the disease and managing oncologists is the nonsuitability of these women for neither hormonal therapy nor adjuvant therapy. Interestingly, just as observed by Tischkowitz et al., triple-negative tumors were also the predominant molecular subtype irrespective of age group of patients or histomorphologic entity. In addition to this, triple-negative tumors have also been associated with BRCA1 mutation. This may explain the poor outcome noted by Dietze et al. among African women with carcinoma of the breast.
HER2 overexpression was the second most common subtype of the ER-negative cases. Adebamowo et al. in an earlier study reported HER2 overexpression as the third most common. However, studies in Sub-Saharan Africa have documented a high proportion of HER2-overexpressed cases ,, with similar studies in Asia. In contrast, Huo et al. and Seshi et al. reported a lower frequency (15% and 25.5%, respectively). The implication for women with this molecular subtype is that their tumors may be more amenable to anti-EGFR-targeted therapy (Trastuzumab). Treatment with adjuvant trastuzumab therapy has been shown to be associated with a 52% increase in disease-free survival and a 33% reduction in risk of death. While this may be a positive, Carey et al. have. however, identified a high risk of early and frequent relapse among patients on this therapy. In Nigeria where resources are less readily available, high cost of trastuzumab may limit access to this drug, just as observed even in developed countries.
The ER-positive cases are composed of LUMA and LUMB tumors. In this study, 35.5% of the cases were ER positive. Although higher than the 2.1% and 11.1% reported by Makanjuola et al. in Lagos  and Banjo et al. in Ogun, respectively, it is close to the percentage (39.5%) reported by Minoza et al. in Maiduguri also in Northern Nigeria but lower than the 80.2% by Adebamowo et al. This wide variation in ER positivity rate is further highlighted by findings from outside Nigeria including Saudi Arabia (74.8%), Eritrea (60%), USA (58%), and Egypt (55.1%). LUMA tumors were common than LUMB in this study mirroring similar report by Minoza et al. also in Northern Nigeria as well as Seshi et al. in Cote d'Ivoire. It however contrasts with that of El Fatemi et al. in Morocco who described a higher frequency of LUMB. ER-positive tumors have been shown to have little response to chemotherapy with LUMB having a poorer prognosis than LUMA. The implication of this in women in Northern Nigeria, though supports use of anti-estrogens (tamoxifen), is counterbalanced by relative poorer response of ER-positive tumors to chemotherapy in addition to high risk of development of resistance to anti-estrogens.
| Conclusion|| |
In the population evaluated, this study has also demonstrated that similar to findings among Blacks, carcinomas of the female breast occur at a younger age than among Caucasians. This study also reaffirms that these tumors, similar to reports from other local studies, are predominantly invasive carcinoma (NST), associated with a high frequency of triple negativity and are also mostly ER negative.
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Conflicts of interest
There are no conflicts of interest.
| References|| |
Aguas F, Martins A, Gomes TP, de Sousa M, Silva DP; Portuguese Menopause Society and Portuguese Gynaecology Society, et al.
Prophylaxis approach to a-symptomatic post-menopausal women: Breast cancer. Maturitas 2005;52 Suppl 1:S23-31.
Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics, 2002. CA Cancer J Clin 2005;55:74-108.
Faratian D, Bartlett J. Predictive markers in breast cancer – The future. Histopathology 2008;52:91-8.
Lakhani SR, Ellis IO, Schnitee SJ, Tan PH, Van de Vijver MJ. World Health Organization Classification of Tumours of the Breast. Lyon, France: International Agency for Research on Cancer (IARC); 2012.
Otu AA, Ekanem IO, Khalil MI, Ekpo MD, Attah EB. Characterization of breast cancer subgroups in an African population. Br J Surg 1989;76:182-4.
Imam MI, Iliyasu Y, Mohammed AZ. Histopathological review of breast tumours in Kano, Nigeria, sub-Sah. Afr J Med 2015;2:47-51.
Adeniji KA. Pathological appraisal of carcinoma of the female breast in Ilorin, Nigeria. Niger Postgrad Med J 1999;6:56-9.
Kouyate M, Koffi KE, Koui BS, Abouna AD, D'horpock AF, Honde M. Histological and epidemiological characteristics of breast cancers in Cote d'Ivoire. Histopathology 2012;1:23.
Elamin A, Ibrahim M, Abuidris D, Mohammed KE, Mohammed SI. Part I: Cancer in Sudan – Burden, distribution and trends breast, gynaecological and prostate cancers. Cancer Med 2015;4:44-56.
Alwan NA. Breast cancer among Iraqi women: Preliminary findings from a regional comparative breast cancer research project. J Glob Oncol 2016;2:255-8.
Dauda AM, Misauno MA, Ojo EO. Histopathological types of breast cancer in Gombe, North Eastern Nigeria: A seven-year review. Afr J Reprod Health 2011;15:109-11.
Titiloye NA, Omoniyi-Esan GO, Adisa AO, Komolafe AO, Afolabi OT, Adelusolo KA. Breast cancer in a Nigerian cohort: Histopathology, Immunohistochemical profile and survival. Postgrad Med J Ghan 2013;2:83-7.
Calvin B, Samaila MO. Histopathological patterns of breast cancer – Experience in a teaching hospital. Histopathology 2012;61:14.
Ebughe GA, Ugare GU, Nnoli MA, Bassey IA, Nwagbara VJ, Udosen JE, et al
. Histological type and tumor grade in Nigerian breast cancer: Relationship to menarche, family history of breast cancer, parity, age at first birth and age at menopause. IOSR J Dent Med Sci 2003;7:58-63.
Sineshaw HM, Gaudet M, Ward EM, Flanders WD, Desantis C, Lin CC, et al.
Association of race/ethnicity, socioeconomic status, and breast cancer subtypes in the national cancer data base (2010-2011). Breast Cancer Res Treat 2014;145:753-63.
Kwong A, Mang OW, Wong CH, Chau WW, Law SC; Hong Kong Breast Cancer Research Group, et al.
Breast cancer in Hong Kong, Southern China: The first population-based analysis of epidemiological characteristics, stage-specific, cancer-specific, and disease-free survival in breast cancer patients: 1997-2001. Ann Surg Oncol 2011;18:3072-8.
Nggada HA, Yawe KD, Abdulazeez J, Khalil MA. Breast cancer burden in Maiduguri, North Eastern Nigeria. Breast J 2008;14:284-6.
Jeje EA, Mofikoya BO, Oku YE. Pattern of breast masses in Lagos: A private health facility review of 189 consecutive patients. Nig Q J Hosp Med 2010;20:38-41.
Clegg-Lamptey J, Hodasi W. A study of breast cancer in Korle Bu teaching hospital: Assessing the impact of health education. Ghana Med J 2007;41:72-7.
Obose AA, Salem OA, Alrabayha M, Alghzawi K. Clinical features and prognostic factors of breast cancer in Jordan. RMJ 2007;32:50-2.
DeSantis C, Ma J, Bryan L, Jemal A. Breast cancer statistics, 2013. CA Cancer J Clin 2014;64:52-62.
Odusanya OO, Tayo OO. Breast cancer knowledge, attitudes and practice among nurses in Lagos, Nigeria. Acta Oncol 2001;40:844-8.
Uche EE. Cancer awareness among a Nigerian population. Trop Doct 1999;29:39-40.
Makanjuola SB, Ayodele SD, Javid FA, Obafunwa JO, Oludara MA, Popoola AO. Breast cancer receptor status assessment and clinicopathological association in Nigerian women: A retrospective analysis. J Cancer Res Ther 2014;2:122-7.
Seshie B, Adu-Aryee NA, Dedey F, Calys-Tagoe B, Clegg-Lamptey JN. A retrospective analysis of breast cancer subtype based on ER/PR and HER2 status in Ghanaian patients at the Korle Bu teaching hospital, Ghana. BMC Clin Pathol 2015;15:14.
Chowdhury N, Pai MR, Lobo FD, Kini H, Varghese R. Interobserver variation in breast cancer grading: A statistical modeling approach. Anal Quant Cytol Histol 2006;28:213-8.
Titloye NA, Foster A, Omoniyi-Esan GO, Komolafe AO, Daramola AO, Adeoye OA, et al.
Histological features and tissue microarray taxonomy of Nigerian breast cancer reveal predominance of the high-grade triple-negative phenotype. Pathobiology 2016;83:24-32.
Minoza KG, Habila KD, Na'aya U, Mustapha Z, Nggada HA. Hormonal and HER2 receptor immunohistochemistry of breast cancer in North-Eastern Nigeria: A preliminary report. IOSR J Dent Med Sci 2016;1:18-23.
El Fatemi H, Chahbouni S, Jayi S, Moumna K, Melhouf MA, Bannani A, et al.
Luminal B tumors are the most frequent molecular subtype in breast cancer of North African women: An immunohistochemical profile study from morocco. Diagn Pathol 2012;7:170.
Adebamowo CA, Famooto A, Ogundiran TO, Aniagwu T, Nkwodimmah C, Akang EE, et al.
Immunohistochemical and molecular subtypes of breast cancer in Nigeria. Breast Cancer Res Treat 2008;110:183-8.
Lund MJ, Trivers KF, Porter PL, Coates RJ, Leyland-Jones B, Brawley OW, et al.
Race and triple negative threats to breast cancer survival: A population-based study in Atlanta, GA. Breast Cancer Res Treat 2009;113:357-70.
Tischkowitz M, Brunet JS, Bégin LR, Huntsman DG, Cheang MC, Akslen LA, et al.
Use of immunohistochemical markers can refine prognosis in triple negative breast cancer. BMC Cancer 2007;7:134.
Zepeda-Castilla EJ, Recinos-Money E, Cuéllar-Hubbe M, Robles-Vidal CD, Maafs-Molina E. Molecular classification of breast cancer. Cir Cir 2008;76:87-93.
Dietze EC, Sistrunk C, Miranda-Carboni G, O'Regan R, Seewaldt VL. Triple-negative breast cancer in African-American women: Disparities versus biology. Nat Rev Cancer 2015;15:248-54.
Banjo AF, Musa O, Tade AO, Ayoade BA, Daramola AO, Abdulkareem FB. Histopathologic characteristics of breast carcinomas at Olabisi Onabanjo University Teaching Hospital Sagamu Ogun state Nigeria. J Health Biomed Sci 2008;7:23-6.
Yarney J, Vanderpuye V, Clegg Lamptey JN. Hormone receptor and HER-2 expression in breast cancers among sub-Saharan African women. Breast J 2008;14:510-1.
Dey S, Boffetta P, Mathews A, Brennan P, Soliman A, Mathew A, et al.
Risk factors according to estrogen receptor status of breast cancer patients in Trivandrum, South India. Int J Cancer 2009;125:1663-70.
Huo D, Ikpatt F, Khramtsov A, Dangou JM, Nanda R, Dignam J, et al.
Population differences in breast cancer: Survey in indigenous African women reveals over-representation of triple-negative breast cancer. J Clin Oncol 2009;27:4515-21.
Romond EH, Perez EA, Bryant J, Suman VJ, Geyer CE Jr., Davidson NE, et al.
Trastuzumab plus adjuvant chemotherapy for operable HER2-positive breast cancer. N
Engl J Med 2005;353:1673-84.
Carey LA, Perou CM, Livasy CA, Dressler LG, Cowan D, Conway K, et al.
Race, breast cancer subtypes, and survival in the Carolina breast cancer study. JAMA 2006;295:2492-502.
Elkablawy MA, Albasry AM, Hussainy AS, Nouh MM, Alhujaily A. Molecular profiling of breast carcinoma in Almadinah, KSA: Immunophenotyping and clinicopathological correlation. Asian Pac J Cancer Prev 2015;16:7819-24.
Tesfamariam A, Roy I. Molecular Biology of Breast Cancer in the Horn of Africa: Case Series – A Pilot Study of Breast Cancer from Eritrea. ISRN Pathology; 2013.
El-Hawary AK, Abbas AS, Elsayed AA, Zalata KR. Molecular subtypes of breast carcinoma in Egyptian women: Clinicopathological features. Pathol Res Pract 2012;208:382-6.
Rastelli F, Crispino S. Factors predictive of response to hormone therapy in breast cancer. Tumori 2008;94:370-83.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
[Table 1], [Table 2], [Table 3], [Table 4]
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