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ORIGINAL ARTICLE
Year : 2019  |  Volume : 10  |  Issue : 2  |  Page : 145-149

Utility of sunflower agar for laboratory detection of Cryptococcus neoformans


1 Department of Medical Microbiology, Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria
2 Department of Medical Microbiology, College of Medicine, University of Jos, Jos, Nigeria
3 Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, College of Health Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria
4 Department of Haematology and Blood Transfusion, Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria

Correspondence Address:
Dr. Sabitu Muhammad Zainu
Department of Medical Microbiology, Usmanu Danfodiyo University Teaching Hospital, Sokoto
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/atp.atp_29_19

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Background: Early diagnosis and management of cryptococcal meningitis is associated with good prognosis and long-term survival. Culture methods have been found to be promising and definitive in the diagnosis of many infectious diseases. We look at the sunflower agar (SFA) cultural method for detection Cryptococcus neoformans, the causative agent of cryptococcal meningitis. Materials and Methods: This is a descriptive cross-sectional study carried out at Jos University Teaching Hospital, Jos Nigeria, to find out the performance of SFA for the detection of C. neoformans. Cerebrospinal fluids (CSFs) of the study subjects were collected, subjected to Indian ink microscopy, inoculated on Sabouraud dextrose agar (SDA) and SFA for identification of C. neoformans. Results: A total of 90 CSF samples were analyzed for the identification of C. neoformans. SFA and SDA were able to confirm 8 (50%) and 7 (43%) of the 16 capsulated yeast cells detected by Indian ink microscopy as C. neoformans. Both media were found to have similar sensitivity (100%), specificity (91.3%), positive predictive value (80%), and negative predictive value (100%) in comparison with Indian ink microscopy. In terms of turnaround time, 6 isolates were identified within an average of 48 h (P = 0.017) by SFA, while SDA detects 2 isolates (P = 0.111) at the stipulated period. Conclusion: SFA can be a good routine conventional culture media for laboratory detection of C. neoformans.


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