|Year : 2018 | Volume
| Issue : 1 | Page : 22-25
The relationship between donor deferral and seropositivity of transfusion-transmissible infections: Implication for transfusion services in the University of Calabar Teaching Hospital, Calabar
Kingsley Akaba1, Benedict Nwogoh2
1 Department of Haematology and Blood Transfusion, University of Calabar Teaching Hospital, Calabar, Cross River State, Nigeria
2 Department of Haematology and Blood Transfusion, University of Benin Teaching Hospital, Benin City, Edo State, Nigeria
|Date of Web Publication||11-Jun-2018|
Dr. Benedict Nwogoh
Department of Haematology and Blood Transfusion, University of Benin Teaching Hospital, Benin City, Edo State
Source of Support: None, Conflict of Interest: None
Introduction: Providing adequate and safe blood has remained a daunting challenge to medical practice, especially in sub-Saharan Africa. Objectives: The objectives of this study were to identify the common reasons for donor deferrals and to determine the seroprevalence of common transfusion-transmissible infections (TTIs) at the University of Calabar Teaching Hospital (UCTH), Calabar. Materials and Methods: This is a retrospective study. Data of blood donors between June 2016 and August 2017 were obtained from the donor registry of the blood bank and analyzed using Microsoft Excel sheet. Results: Reactive test for TTIs and suboptimal packed cell volume (PCV) were the most common reasons for donor deferrals in UCTH, Calabar. A total of 12.3% of potential donors were deferred mainly due to positive screening test for TTIs and suboptimal PCV. The seroprevalence for hepatitis B, hepatitis C, human immunodeficiency viruses, and syphilis was 2.9%, 2.8%, 1.5%, and 2.5%, respectively. Conclusion: Seropositivity for TTIs and suboptimal hematocrit levels were the major reasons for donor deferrals in UCTH, Calabar.
Keywords: Donor deferrals, seroprevalence, transfusion-transmissible infections
|How to cite this article:|
Akaba K, Nwogoh B. The relationship between donor deferral and seropositivity of transfusion-transmissible infections: Implication for transfusion services in the University of Calabar Teaching Hospital, Calabar. Ann Trop Pathol 2018;9:22-5
|How to cite this URL:|
Akaba K, Nwogoh B. The relationship between donor deferral and seropositivity of transfusion-transmissible infections: Implication for transfusion services in the University of Calabar Teaching Hospital, Calabar. Ann Trop Pathol [serial online] 2018 [cited 2019 Sep 22];9:22-5. Available from: http://www.atpjournal.org/text.asp?2018/9/1/22/234148
| Introduction|| |
The attainment of donor blood sufficiency and safety has remained an onerous task in our environment. Voluntary blood donation has been adjudged the safest source of donor blood procurement; however, they are limited and cannot sustain the blood requirement of medical practice in most facilities in Nigeria. Most centers have resorted to replacement and paid donors in some instances to meet the increased demand for blood and blood products., In a number of cases, paid donors may even disguise themselves as replacement donors. The inability to maximize every unit of donated blood by separating it into component further worsens the challenge of providing adequate blood.
The nonvoluntary donor blood procurement is saddled with an increased risk of transfusion-transmissible infections (TTIs). This is worsened in settings like ours where improved safety practices such as use of leukodepleted blood, blood product irradiation, pathogen inactivation techniques, and nucleic acid testing methods are lacking.
This study seeks to analyze the blood donation practice in the University of Calabar Teaching Hospital (UCTH), with a view to identify reasons for donor deferrals, the seroprevalence of TTIs in our donor populace. This is intended to make suggestions that will increase our donor blood procurement.
| Materials and Methods|| |
This was a retrospective study conducted at UCTH, Calabar. Data of blood donors between June 2016 and August 2017 were obtained from the donor registry for analysis. The facility is mainly dependent on replacement donations and voluntary donor blood sourced mainly from the national blood transfusion services Calabar unit. The blood donation eligibility criteria include age range of 18–60 years, weight >50 kg, absence of any high-risk behavior, medical or physiological status that may endanger the donor or recipient, and packed cell volume (PCV) of 40% irrespective of the sex. The donors were screened for four common TTIs, namely, hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus (HIV), and Treponema pallidum (syphilis) using enzyme-linked immunosorbent assay-based rapid kits. Two different kits were used for the HIV screening, and if any was reactive, the donor was deferred. The donors were screened for ABO and rhesus D blood group status. Donors who were not group compatible with potential recipient may be bled while a group compatible unit is given to potential recipient.
Data obtained include the number of donors screened during the study period, their sex, age PCV, screening outcome for TTIs, number of donors declared fit, and bleed. Those not fit to donate are counseled based on the identified reason for deferral. Temporary deferred donors such as those due to low PCV were counseled on how to improve their PCV while those with very low PCV were referred to the hematologist for treatment. Those deferred on account of TTI were referred to the appropriate clinic for management.
The results were analyzed using Microsoft Excel sheet and expressed in frequencies and percentages.
| Results|| |
A total of 2695 donors were screened during the study period (June 2016–August 2017) giving an average of 192 donors per month. Majority (53.3%) of the donors were within the age range of 29–38 years as shown in [Figure 1]. Two thousand two hundred and eighty-one of them were males (84.6%) with a male-to-female ratio of 7:1 as shown in [Figure 2].
Replacement donors account for the highest proportion of donors and account for 1198 (44.5%), and this represented the highest proportion of blood donors. Voluntary donors were the least proportion, accounting for 599 (22.2%) as shown in [Table 1]. Two thousand three hundred and sixty-two (87.9%) of the donors were found fit to donate as shown in [Figure 3].
Three hundred and thirty-two (12.3%) donors were unfit for blood donation and were deferred. Reasons for deferral include suboptimal PCV alone in 71 (21.4%), seropositivity for TTI in 186 (56.0%) donors, and combination of suboptimal PCV and TTIs in 75 (22.6%)
Seventy-five donors tested positive for hepatitis B surface antigen (HBsAg) giving a seroprevalence rate of 2.8%, 78 (2.9%) donors were positive for hepatitis C antibodies, 67 (2.5%) were positive for venereal disease research laboratory (Treponema pallidum antibodies), and 44 (1.5%) were positive for HIV antibodies in [Table 2].
[Table 3] shows the distribution of seropositivity for TTI based on age group distribution of donors. The highest proportion of TTIs was reported in donors between 29 and 48 years.
|Table 3: Age group distribution of transfusion-transmissible infection in donors|
Click here to view
| Discussion|| |
This study has shown that UCTH, Calabar, has predominantly a male donor population accounting for 84.6%. This is similar to the observations of most other researchers in different parts of Nigeria and the world.,,,, The male-to-female ratio of donor populace may vary from one location to another, but it is worth mentioning that the male-to-female ratio observed in this study is astronomically high compared to other African-based studies ,,, and the WHO global report. However, this is consistent with most studies in Nigeria that have reported values in the range of 85.1%–99.6%.,,,, The reason for male dominance is partly because a common minimum hematocrit (39%) requirement is adopted for both male and female intending donors in Calabar. The universally acceptable hemoglobin requirement for blood donation is 13 g/dl for males and 12 g/dl for females (This translates to a PCV of 39% and 36% for males and females, respectively). The adoption of a common hematocrit for both sexes may grossly limit females from donating bloods and over time may have discouraged female donors. Burden of the regular female menstrual cycle, childbirth, and suboptimal nutrition on the hematologic system of women further limits their capacity to attain sufficient hematocrit for blood donation.
Majority of the donors were in the age range of 29–38 years (53.3%). This differs from the WHO report which showed 45% of donors were aged 25 years or less  but similar to that of a study done in South West Nigeria by Buseri et al. This may reflect the inadequate sensitization program on the blood donation in early adulthood. It may also reflect the type/goal of the donors which predominantly were replacement and remunerated donors in this setting.
Contrary to the WHO directives that blood donation should be completely voluntary, the center recorded only 22.2% of voluntary donation during the period. This proportion is grossly insufficient to meet the blood demands of the hospital. In our setting, replacement and remunerated donations still play important roles in addressing blood insufficiency and should not be jettisoned in a hurry despite the inherent risk associated with them. While other measures to increase blood sufficiencies such as blood component therapy and rational blood use are being implemented, there should be increased campaign for voluntary donations. The replacement/remunerated donor population should be counseled and possibly converted and retained as voluntary donors.
Seropositivity for the major TTI (HIV, HBsAg, HCV antibodies, and syphilis) and suboptimal hemoglobin levels were the major reasons for donor deferrals. The seroprevalence of HBsAg was found to be 2.8%. The prevalence rate of HBsAg obtained from this study was lower than rates reported in a number of studies in other regions of Nigeria ,,, but higher than rates reported in some other countries. The relatively low value in this study should be due to high vaccination rates and improved behavioral practices that reduce risk of transmission in the general population. It is important to establish a protocol of referral and management of seropositive individuals.
The seroprevalence of HCV antibodies was 2.0%. Similar rate was reported by Koate et al. and Wansbrough-Jones et al. (2.8%) in Port Harcourt and Ghana, respectively. Another Port Harcourt-based study reported higher value of 5.0%. Other institutional-based study reported rates ranging from 6% to 12.3%.,, Variation in seroprevalence rate may in part be due to varying precision and accuracy of the assay kits. Very sensitive kits tend to report higher values. With increased awareness of the disease, it is expected that rates will decline hence older studies conducted when awareness was relatively low recorded higher prevalence. The youths (18–48 years' age group) had the highest rate. This finding is in agreement with studies of Baba et al. and Ejele et al. This age group is more energetic and tends to exhibit high-risk behavior (prostitution, drug use, etc.). This observation is perturbing since the most productive and economically viable age group of the population is the most affected. There is an urgent need for intensification of prevention programs to control such high-risk behaviors.
The seroprevalence of HIV infection was 1.5% with the highest burden among those between 18 and 48 years. This may have negative implications for the labor force, productivity, and economy of the state. This rate is lower than those reported by other studies on blood donors in Nigeria that recorded rates ranging from 0.08% in Ile-Ife to 10.6% in Aba., The prevalence rate tends to correspond with the state prevalence as reported in the National HIV/AIDS Reproductive Health Survey. The survey reported prevalence rates ranging from 0.9% in Ekiti to as much as 15.2% in Rivers. Cross River State was reported to have 4.4%. The multiple kit algorithm screening method adopted by the center may account for the relatively low rate observed as it helps to minimize false-positive result.
We found a seroprevalence rate 2.5% for syphilis among the apparently healthy intending blood donors. This is lower than 3.6% reported in Maiduguri  and 12.4% in Ilorin  but higher than 0.1% reported by Ejele et al. in Port Harcourt, and Nwogoh et al. reported 1.19% in Benin, southern Nigeria. There is geographical variation in prevalence of these infections. The risk of transmission of syphilis in donor blood higher in transfusion of freshly donated blood but declines after 48 h of refrigeration under optimal temperature for red cell storage (2 - 80C).
The study has some limitations. All the possible reasons for donor deferral may not have being captured. This is due to information bias due to inadequate recording.
| Conclusion|| |
Seropositivity for TTIs and suboptimal hematocrit are the major reasons for deferral of potential donors in UCTH, Calabar. There is a high proportion of remunerated and replacement donors in our donor pool which may account for the present seroprevalence rate recorded.
There is a need to educate and encourage the donor population, especially family replacement and remunerated donors to adopt voluntary donation practice to improve blood safety. The hospital should adopt other measures such as rationalizing blood use, production and use of blood component, and developing a guideline for transfusion practices in the hospital. The hospital should revise the current practice of a uniform hematocrit limit for blood donors to encourage female donors.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Erhabor O, Adias TC. Challenges to meeting the future blood transfusion requirements in sub Saharan Africa. The need for the development of alternatives to allogenic blood. J Blood Med 2010;2:1-15.
Federal Ministry of Health Nigeria. Nigerian National Blood Policy. Abuja, Nigeria: National Blood Transfusion Service; 2005.
Koistinen J. Safe blood: The WHO sets out its principles. AIDS Anal Afr 1992;2:4-6.
Tagny CT, Diarra A, Yahaya R, Hakizimana M, Nguessan A, Mbensa G, et al.
Characteristics of blood donors and donated blood in sub-saharan francophone africa. Transfusion 2009;49:1592-9.
Agbovi KK, Kolou M, Fétéké L, Haudrechy D, North ML, Ségbéna AY, et al.
Knowledge, attitudes and practices about blood donation. A sociological study among the population of lomé in togo. Transfus Clin Biol 2006;13:260-5.
Nébié KY, Olinger CM, Kafando E, Dahourou H, Diallo S, Kientega Y, et al.
Lack of knowledge among blood donors in burkina faso (West Africa); Potential obstacle to transfusion security. Transfus Clin Biol 2007;14:446-52.
Allain JP, Sarkodie F, Boateng P, Asenso K, Kyeremateng E, Owusu-Ofori S, et al.
A pool of repeat blood donors can be generated with little expense to the blood center in sub-saharan africa. Transfusion 2008;48:735-41.
Buseri FI, Muhibi MA, Jeremiah ZA. Sero-epidemiology of transfusion-transmissible infectious diseases among blood donors in Osogbo, south-west Nigeria. Blood Transfus 2009;7:293-9.
Nwankwo E, Mamodu I, Umar I, Musa B, Adeleke S. Seroprevalence of major blood-borne infections among blood donors in Kano, Nigeria. Turk J Med Sci 2012;42:337-41.
Baye G, Mengistu Y. The prevalence of HBV, HCV and malaria parasites among blood donors in Amhara and Tigray regional states. Ethiop J Health Dev 2007;22:3-7.
Ismail MA, Amirthalingam R. Seroprevalence of HBV, HCV and HIV infectivity among blood donors in ibn sina teaching hospital in sirt region of Libya. Int J Med Res Health Sci 2013;2:816-22.
Baba MM, Hassan AW, Gashau W. Prevalence of hepatitis B antigenaemia and human immunodeficiency virus in blood donors in Maidugiri, Nigeria. Niger J Med 2000;9:10-2.
WHO Expert Group. Expert consensus statement on achieving self-sufficiency in safe blood and blood products, based on voluntary non-remunerated blood donation (VNRBD). Vox Sang 2012;103:337-42.
Mustapha SK, Jibrin YB. The prevalence of hepatitis B surface antigenaemia in patients with human immunodeficiency virus (HIV) infection in Gombe, Nigeria. Ann Afr Med 2004;3:10-2.
Ampofo W, Nii-Trebi N, Ansah J, Abe K, Naito H, Aidoo S, et al.
Prevalence of blood-borne infectious diseases in blood donors in ghana. J Clin Microbiol 2002;40:3523-5.
Qowaider SR, Ali MS, Moftah SA, Khaled FA. Prevalence of HBV and HCV infections among blood donors in Northeast Libya. Int Blood Res Rev 2007;7:1-5.
Koate BB, Buseri FI, Jeremiah ZA. Seroprevalence of hepatitis C virus among blood donors in Rivers State, Nigeria. Transfus Med 2005;15:449-51
Wansbrough-Jones MH, Frimpong E, Cant B, Harris K, Evans MR, Teo CG, et al.
Prevalence and genotype of hepatitis C virus infection in pregnant women and blood donors in ghana. Trans R Soc Trop Med Hyg 1998;92:496-9.
Halim NK, Ajayi OI. Risk factors and seroprevalence of hepatitis C antibody in blood donors in nigeria. East Afr Med J 2000;77:410-2.
Egah DZ, Mandong BM, Iya D, Gomwalk NE, Audu ES, Banwat EB, et al
. Hepatitis C virus antibodies among blood donors in Jos, Nigeria. Ann Afr Med 2004;3:35-7.
Mutimer DJ, Olomu A, Skidmore S, Olomu N, Ratcliffe D, Rodgers B, et al.
Viral hepatitis in nigeria – sickle-cell disease and commercial blood donors. QJM 1994;87:407-11.
Ejele OA, Erhabor O, Nwauche CA. Trends in the prevalence of some transfusion-transmissible infections among blood donors in Port Harcourt, Nigeria. Haema 2005;8:273-7.
Durosinmi MA, Ndububa DA, Alabi OA, Soyinka OO. Prevalence of HIV-1 and HbsAg in normal blood donors in Ile-Ife, Nigeria. Niger Med J 1992;21:138-40.
Amadi AN, Mba LE. Distribution of HIV infection in Abia State, Nigeria. Niger J Med Invest Pract 2001;2:38-40.
Nwabuisi C, Aderinola CI, Ibegbulam OG. The seroprevalence of syphilis in unscreened and unstored blood transfused in Ilorin, Nigeria. Medipharm Med J 2005;2:7-9.
Nwogoh B, Adewoyin AS, Bazuaye GN, Enosolease ME. Seroprevalence of Treponema pallidum
in donor blood at the university of Benin teaching hospital, Benin city. Ann Biomed Sci 2013;12:8-12.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3]